Coding

Part:BBa_K3468032:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase V149I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. V149I was designed by searching for suitable sites around K148 for mutation.


Source

Ideonalla sakaiensis

References